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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
06/10/2022 |
Actualizado : |
06/10/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
GIANNITTI, F.; SILVEIRA, C.S.; BULLOCK, H.; BERON, M.; FERNÁNDEZ-CIGANDA, S.; BENÍTEZ-GALEANO, M.J.; RODRÍGUEZ-OSORIO, N.; SILVA-FLANNERY, L.; PERDOMO, T.; CABRERA, A.; PUENTES, R.; COLINA, R.; RITTER, J.M.; CASTELLS, M. |
Afiliación : |
FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; HANNAH BULLOCK, Synergy America Inc., Atlanta, GA 30329, USA; MARINA MAURENTE BERON, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SOFÍA FERNÁNDEZ-CIGANDA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA JOSÉ BENÍTEZ-GALEANO, Unidad de Genómica y Bioinformática, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto 50000, Uruguay; NÉLIDA RODRÍGUEZ-OSORIO, Unidad de Genómica y Bioinformática, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto 50000, Uruguay; LUCIANA SILVA-FLANNERY, Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA 30329, USA; TERESITA YISELL PERDOMO TORRES, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANDRÉS CABRERA, Facultad de Veterinaria, Universidad de la República, Montevideo 13000, Uruguay; Laboratorio de Interacciones Hospedero-Patógeno, Institut Pasteur de Montevideo, Montevideo 11400, Uruguay; RODRIGO PUENTES, Facultad de Veterinaria, Universidad de la República, Montevideo 13000, Uruguay; RODNEY COLINA, Laboratorio de Virología Molecular, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto 50000, Uruguay; JANA M. RITTER, Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA 30329, USA; MATÍAS CASTELLS, Laboratorio de Virología Molecular, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto 50000, Uruguay. |
Título : |
Bovine Polyomavirus-1 (Epsilonpolyomavirus bovis): An emerging fetal pathogen of cattle that causes renal lesions resembling Polyomavirus-associated nephropathy of humans. |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
Viruses, 2022; 14 (9): 2042. OPEN ACCESS. doi: https://doi.org/10.3390/v14092042 |
ISSN : |
1999-4915 |
DOI : |
10.3390/v14092042 |
Idioma : |
Inglés |
Notas : |
Article history: Received 12 August 2022; Revised 8 September 2022: Accepted 9 September 2022; Published 14 September 2022.
Academic Editors: Fernando Bauermann and Mayara Maggioli.
Correspondence authors: Giannitti, F.; Plataforma de Investigación en Salud Animal, Instituto Nacional de Investigación Agropecuaria (INIA), Estación Experimental La Estanzuela, Colonia, Uruguay; email:fgiannitti@inia.org.uy - Castells, M.; Laboratorio de Virología Molecular, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto, Uruguay; email:matiascastellsbauer@gmail.com --
Funding: Instituto Nacional de Investigación Agropecuaria (INIA), grant PL_27 N-23398. -- This article belongs to the Special Issue Pathogenesis and Host Responses to Viral Diseases in Livestock Species: https://www.mdpi.com/journal/viruses/special_issues/pathogenesis_livestock |
Contenido : |
ABSTRACT.- Bovine polyomavirus-1 (BoPyV-1, Epsilonpolyomavirus bovis) is widespread in cattle and has been detected in commercialized beef at supermarkets in the USA and Germany. BoPyV-1 has been questioned as a probable zoonotic agent with documented increase in seropositivity in people exposed to cattle. However, to date, BoPyV-1 has not been causally associated with pathology or disease in any animal species, including humans. Here we describe and illustrate pathological findings in an aborted bovine fetus naturally infected with BoPyV-1, providing evidence of its pathogenicity and probable abortigenic potential. Our results indicate that: (i) BoPyV-1 can cause severe kidney lesions in cattle, including tubulointerstitial nephritis with cytopathic changes and necrosis in tubular epithelial cells, tubular and interstitial inflammation, and interstitial fibroplasia; (ii) lesions are at least partly attributable to active viral replication in renal tubular epithelial cells, which have abundant intranuclear viral inclusions; (iii) BoPyV-1 large T (LT) antigen, resulting from early viral gene expression, can be detected in infected renal tubular epithelial cells using a monoclonal antibody raised against Simian Virus-40 polyomavirus LT antigen; and (iv) there is productive BoPyV-1 replication and virion assembly in the nuclei of renal tubular epithelial cells, as demonstrated by the ultrastructural observation of abundant arrays of viral particles with typical polyomavirus morphology. Altogether, these lesions resemble the "cytopathic-inflammatory pathology pattern" proposed in the pathogenesis of Human polyomavirus-1-associated nephropathy in immunocompromised people and kidney allograft recipients. Additionally, we sequenced the complete genome of the BoPyV-1 infecting the fetus, which represents the first whole genome of a BoPyV-1 from the Southern Hemisphere. Lastly, the BoPyV-1 strain infecting this fetus was isolated, causing a cytopathic effect in Madin-Darby bovine kidney cells. We conclude that BoPyV-1 is pathogenic to the bovine fetus under natural circumstances. Further insights into the epidemiology, biology, clinical relevance, and zoonotic potential of BoPyV-1 are needed. © 2022 by the authors. MenosABSTRACT.- Bovine polyomavirus-1 (BoPyV-1, Epsilonpolyomavirus bovis) is widespread in cattle and has been detected in commercialized beef at supermarkets in the USA and Germany. BoPyV-1 has been questioned as a probable zoonotic agent with documented increase in seropositivity in people exposed to cattle. However, to date, BoPyV-1 has not been causally associated with pathology or disease in any animal species, including humans. Here we describe and illustrate pathological findings in an aborted bovine fetus naturally infected with BoPyV-1, providing evidence of its pathogenicity and probable abortigenic potential. Our results indicate that: (i) BoPyV-1 can cause severe kidney lesions in cattle, including tubulointerstitial nephritis with cytopathic changes and necrosis in tubular epithelial cells, tubular and interstitial inflammation, and interstitial fibroplasia; (ii) lesions are at least partly attributable to active viral replication in renal tubular epithelial cells, which have abundant intranuclear viral inclusions; (iii) BoPyV-1 large T (LT) antigen, resulting from early viral gene expression, can be detected in infected renal tubular epithelial cells using a monoclonal antibody raised against Simian Virus-40 polyomavirus LT antigen; and (iv) there is productive BoPyV-1 replication and virion assembly in the nuclei of renal tubular epithelial cells, as demonstrated by the ultrastructural observation of abundant arrays of viral particles with typical polyomavirus mor... Presentar Todo |
Palabras claves : |
Abortion; Cattle; Emerging diseases; Epsilonpolyomavirus bovis; Nephropathy; Next generation sequencing; Pathology; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; Polyomavirus; Reproductive diseases; Viral diseases. |
Asunto categoría : |
L20 Ecología animal |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16810/1/viruses-14-02042-v2.pdf
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Marc : |
LEADER 04533naa a2200445 a 4500 001 1063641 005 2022-10-06 008 2022 bl uuuu u00u1 u #d 022 $a1999-4915 024 7 $a10.3390/v14092042$2DOI 100 1 $aGIANNITTI, F. 245 $aBovine Polyomavirus-1 (Epsilonpolyomavirus bovis)$bAn emerging fetal pathogen of cattle that causes renal lesions resembling Polyomavirus-associated nephropathy of humans.$h[electronic resource] 260 $c2022 500 $aArticle history: Received 12 August 2022; Revised 8 September 2022: Accepted 9 September 2022; Published 14 September 2022. Academic Editors: Fernando Bauermann and Mayara Maggioli. Correspondence authors: Giannitti, F.; Plataforma de Investigación en Salud Animal, Instituto Nacional de Investigación Agropecuaria (INIA), Estación Experimental La Estanzuela, Colonia, Uruguay; email:fgiannitti@inia.org.uy - Castells, M.; Laboratorio de Virología Molecular, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto, Uruguay; email:matiascastellsbauer@gmail.com -- Funding: Instituto Nacional de Investigación Agropecuaria (INIA), grant PL_27 N-23398. -- This article belongs to the Special Issue Pathogenesis and Host Responses to Viral Diseases in Livestock Species: https://www.mdpi.com/journal/viruses/special_issues/pathogenesis_livestock 520 $aABSTRACT.- Bovine polyomavirus-1 (BoPyV-1, Epsilonpolyomavirus bovis) is widespread in cattle and has been detected in commercialized beef at supermarkets in the USA and Germany. BoPyV-1 has been questioned as a probable zoonotic agent with documented increase in seropositivity in people exposed to cattle. However, to date, BoPyV-1 has not been causally associated with pathology or disease in any animal species, including humans. Here we describe and illustrate pathological findings in an aborted bovine fetus naturally infected with BoPyV-1, providing evidence of its pathogenicity and probable abortigenic potential. Our results indicate that: (i) BoPyV-1 can cause severe kidney lesions in cattle, including tubulointerstitial nephritis with cytopathic changes and necrosis in tubular epithelial cells, tubular and interstitial inflammation, and interstitial fibroplasia; (ii) lesions are at least partly attributable to active viral replication in renal tubular epithelial cells, which have abundant intranuclear viral inclusions; (iii) BoPyV-1 large T (LT) antigen, resulting from early viral gene expression, can be detected in infected renal tubular epithelial cells using a monoclonal antibody raised against Simian Virus-40 polyomavirus LT antigen; and (iv) there is productive BoPyV-1 replication and virion assembly in the nuclei of renal tubular epithelial cells, as demonstrated by the ultrastructural observation of abundant arrays of viral particles with typical polyomavirus morphology. Altogether, these lesions resemble the "cytopathic-inflammatory pathology pattern" proposed in the pathogenesis of Human polyomavirus-1-associated nephropathy in immunocompromised people and kidney allograft recipients. Additionally, we sequenced the complete genome of the BoPyV-1 infecting the fetus, which represents the first whole genome of a BoPyV-1 from the Southern Hemisphere. Lastly, the BoPyV-1 strain infecting this fetus was isolated, causing a cytopathic effect in Madin-Darby bovine kidney cells. We conclude that BoPyV-1 is pathogenic to the bovine fetus under natural circumstances. Further insights into the epidemiology, biology, clinical relevance, and zoonotic potential of BoPyV-1 are needed. © 2022 by the authors. 653 $aAbortion 653 $aCattle 653 $aEmerging diseases 653 $aEpsilonpolyomavirus bovis 653 $aNephropathy 653 $aNext generation sequencing 653 $aPathology 653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL 653 $aPolyomavirus 653 $aReproductive diseases 653 $aViral diseases 700 1 $aSILVEIRA, C.S. 700 1 $aBULLOCK, H. 700 1 $aBERON, M. 700 1 $aFERNÁNDEZ-CIGANDA, S. 700 1 $aBENÍTEZ-GALEANO, M.J. 700 1 $aRODRÍGUEZ-OSORIO, N. 700 1 $aSILVA-FLANNERY, L. 700 1 $aPERDOMO, T. 700 1 $aCABRERA, A. 700 1 $aPUENTES, R. 700 1 $aCOLINA, R. 700 1 $aRITTER, J.M. 700 1 $aCASTELLS, M. 773 $tViruses, 2022; 14 (9): 2042. OPEN ACCESS. doi: https://doi.org/10.3390/v14092042
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Registro original : |
INIA Las Brujas (LB) |
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Biblioteca (s) : |
INIA Tacuarembó. |
Fecha actual : |
06/11/2019 |
Actualizado : |
06/11/2019 |
Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
Autor : |
MONTOSSI, F.; CAZZULI, F. |
Afiliación : |
FABIO MARCELO MONTOSSI PORCHILE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FIORELLA CARLA CAZZULI ALBA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Ejemplos empresariales y asociativos de marcas de calidad: Uruguay [Associative business examples and quality brands: Uruguay]. |
Fecha de publicación : |
2019 |
Fuente / Imprenta : |
In: TEIXEIRA, A.; SAÑUDO, C. (Coord.). Marcas de calidad de carne en Iberoamerica: Caribe, Sudamerica y Peninsula Iberica. Congreso Iberoamericano de Marcas de Calidad de Carne y de Productos Cárnicos, 24-25 de octubre, Bragança, Portugal, 2019. Bragança (Portugal): RED MARCARNE, 2019. |
Páginas : |
p. 181-190 |
Idioma : |
Español |
Contenido : |
Resumen: En un marco de intensificación sostenible de la ganadería nacional, Uruguay viene impulsando una estrategia de diferenciación y agregado de
valor de productos y procesos desde una visión integral ?del campo al plato del consumidor?. En este contexto, existen una serie de experiencias exitosas
a destacar entre los diferentes actores de la cadena cárnica del Uruguay. Este artículo se focaliza en describir, analizar y reflexionar sobre algunos
casos exitosos de emprendimientos empresariales asociativos del desarrollo de marcas y/o procesos de certificaciones que permitieron consolidar
agronegocios ganaderos innovadores y competitivos. Entre los factores en común que presentan estas iniciativas, se destacan: orientadas a nichos de
mercado de alto valor, experiencia grupal previa, protocolo y certificación, asistencia técnica, gobernanza y procesos de gestión que favorecen la
confianza y la transparencia, una clara vocación de anticipar, acompañar y capturar las oportunidades que ofrece el mercado // Summary: In a framework of sustainable intensification of national livestock, Uruguay has been promoting a strategy of differentiation and added value of products
and processes from a comprehensive vision “from the field to the plate of the consumer”. In this context, there are series of successful experiences to
be highlighted among the different actors of the Uruguayan meat chain of Uruguay. This article focuses on describing, analysing and reflecting on some
successful cases of associative entrepreneurial ventures of brand development and/or certification processes that allowed the consolidation of innovative and competitive livestock agribusinesses. Among the common factors presented by these initiatives, we highlight: oriented to high-value market niches, previous working group experience, protocol and certification process, technical assistance, governance and management processes that favour trust and transparency, a clear vocation for anticipation, accompany and capture the opportunities offered by the market. MenosResumen: En un marco de intensificación sostenible de la ganadería nacional, Uruguay viene impulsando una estrategia de diferenciación y agregado de
valor de productos y procesos desde una visión integral ?del campo al plato del consumidor?. En este contexto, existen una serie de experiencias exitosas
a destacar entre los diferentes actores de la cadena cárnica del Uruguay. Este artículo se focaliza en describir, analizar y reflexionar sobre algunos
casos exitosos de emprendimientos empresariales asociativos del desarrollo de marcas y/o procesos de certificaciones que permitieron consolidar
agronegocios ganaderos innovadores y competitivos. Entre los factores en común que presentan estas iniciativas, se destacan: orientadas a nichos de
mercado de alto valor, experiencia grupal previa, protocolo y certificación, asistencia técnica, gobernanza y procesos de gestión que favorecen la
confianza y la transparencia, una clara vocación de anticipar, acompañar y capturar las oportunidades que ofrece el mercado // Summary: In a framework of sustainable intensification of national livestock, Uruguay has been promoting a strategy of differentiation and added value of products
and processes from a comprehensive vision “from the field to the plate of the consumer”. In this context, there are series of successful experiences to
be highlighted among the different actors of the Uruguayan meat chain of Uruguay. This article focuses on describing, analysing and reflecting on some
successful ca... Presentar Todo |
Palabras claves : |
ASSOCIATIVE VENTURES; BOVINOS; BRANDS; CATTLE; EMPRENDIMIENTOS ASOCIATIVOS; MARCAS; MEAT; URUGUAY. |
Asunto categoría : |
Q01 Ciencia y tecnología de los alimentos |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/13758/1/Cazzuli-y-Montossi-2019-1.pdf
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Marc : |
LEADER 03006naa a2200241 a 4500 001 1060384 005 2019-11-06 008 2019 bl uuuu u00u1 u #d 100 1 $aMONTOSSI, F. 245 $aEjemplos empresariales y asociativos de marcas de calidad$bUruguay [Associative business examples and quality brands: Uruguay].$h[electronic resource] 260 $c2019 300 $ap. 181-190 520 $aResumen: En un marco de intensificación sostenible de la ganadería nacional, Uruguay viene impulsando una estrategia de diferenciación y agregado de valor de productos y procesos desde una visión integral ?del campo al plato del consumidor?. En este contexto, existen una serie de experiencias exitosas a destacar entre los diferentes actores de la cadena cárnica del Uruguay. Este artículo se focaliza en describir, analizar y reflexionar sobre algunos casos exitosos de emprendimientos empresariales asociativos del desarrollo de marcas y/o procesos de certificaciones que permitieron consolidar agronegocios ganaderos innovadores y competitivos. Entre los factores en común que presentan estas iniciativas, se destacan: orientadas a nichos de mercado de alto valor, experiencia grupal previa, protocolo y certificación, asistencia técnica, gobernanza y procesos de gestión que favorecen la confianza y la transparencia, una clara vocación de anticipar, acompañar y capturar las oportunidades que ofrece el mercado // Summary: In a framework of sustainable intensification of national livestock, Uruguay has been promoting a strategy of differentiation and added value of products and processes from a comprehensive vision “from the field to the plate of the consumer”. In this context, there are series of successful experiences to be highlighted among the different actors of the Uruguayan meat chain of Uruguay. This article focuses on describing, analysing and reflecting on some successful cases of associative entrepreneurial ventures of brand development and/or certification processes that allowed the consolidation of innovative and competitive livestock agribusinesses. Among the common factors presented by these initiatives, we highlight: oriented to high-value market niches, previous working group experience, protocol and certification process, technical assistance, governance and management processes that favour trust and transparency, a clear vocation for anticipation, accompany and capture the opportunities offered by the market. 653 $aASSOCIATIVE VENTURES 653 $aBOVINOS 653 $aBRANDS 653 $aCATTLE 653 $aEMPRENDIMIENTOS ASOCIATIVOS 653 $aMARCAS 653 $aMEAT 653 $aURUGUAY 700 1 $aCAZZULI, F. 773 $tIn: TEIXEIRA, A.; SAÑUDO, C. (Coord.). Marcas de calidad de carne en Iberoamerica: Caribe, Sudamerica y Peninsula Iberica. Congreso Iberoamericano de Marcas de Calidad de Carne y de Productos Cárnicos, 24-25 de octubre, Bragança, Portugal, 2019. Bragança (Portugal): RED MARCARNE, 2019.
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